Difference between revisions of "LX Reference Herzog 2012"
(Created page with '====Queries, spectra and results==== All data from the experiments can be found here: [https://wiki.mpi-cbg.de/wiki/lipidx/images/3/3f/Mass_Spectra_used_in_the_article.zip Mass_...') |
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For the acquisition in '''negative mode''' of samples with QStar with data-dependent acquisition (DDA), LTQ Orbitrap Velos with DDA mode and TSQ Vantage with precursor ion scan (PIS) mode (see Figure 3 of the article): | For the acquisition in '''negative mode''' of samples with QStar with data-dependent acquisition (DDA), LTQ Orbitrap Velos with DDA mode and TSQ Vantage with precursor ion scan (PIS) mode (see Figure 3 of the article): | ||
| − | * [[ | + | * [[media:PE_negative_FAS.mfql | PE_negative_FAS.mfql]] |
| − | * [[ | + | * [[media:PG_negative_FAS.mfql | PG_negative_FAS.mfql]] |
For the acquisition in '''positive mode''' of samples with TSQ Vantage with neutral loss scan (PIS) mode (see Figure 4 of the article) for the loss of head groups of PE and PG: Δ m/z 141.02 for [M+H]+ molecular ions of PE and Δ m/z 189.04 for ammonium adducts [M+NH4]+ of PG. Because the actual spectra are stored as precursor ion scan spectra, the queries are customized to PIS: | For the acquisition in '''positive mode''' of samples with TSQ Vantage with neutral loss scan (PIS) mode (see Figure 4 of the article) for the loss of head groups of PE and PG: Δ m/z 141.02 for [M+H]+ molecular ions of PE and Δ m/z 189.04 for ammonium adducts [M+NH4]+ of PG. Because the actual spectra are stored as precursor ion scan spectra, the queries are customized to PIS: | ||
| − | * [[ | + | * [[media:PE_positive_NLS-_-PIS.mfql]] |
| − | * [[ | + | * [[media:PG_positive_NLS-_-PIS.mfql]] |
Revision as of 15:07, 15 March 2012
Queries, spectra and results
All data from the experiments can be found here: Mass_Spectra_used_in_the_article.zip
Materials and Methods
Please have a look into the article for a detailed description of the experiments: PLoS One 7(1): e29851
MFQL queries
For the acquisition in negative mode of samples with QStar with data-dependent acquisition (DDA), LTQ Orbitrap Velos with DDA mode and TSQ Vantage with precursor ion scan (PIS) mode (see Figure 3 of the article):
For the acquisition in positive mode of samples with TSQ Vantage with neutral loss scan (PIS) mode (see Figure 4 of the article) for the loss of head groups of PE and PG: Δ m/z 141.02 for [M+H]+ molecular ions of PE and Δ m/z 189.04 for ammonium adducts [M+NH4]+ of PG. Because the actual spectra are stored as precursor ion scan spectra, the queries are customized to PIS:
