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| − | ====Queries, spectra and results==== | + | ==Survival strategies of a sterol auxotroph== |
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| − | ====Materials and Methods====
| + | ===Materials and Methods=== |
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| − | Top-down analysis of the fly total membrane lipidome was performed on an LTQ Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) equipped with a NanoMate robotic nanoflow ion source (Advion BioSciences, Ithaca, NJ, USA) as described previously (Schwudke et al., 2007a). MS/MS experiments on the LTQ Orbitrap and the QSTAR Pulsar i (MDS Sciex, Toronto, Canada) mass spectrometers were performed as described previously (Schwudke et al., 2007b). Absolute quantification of ceramides and hexosyl-ceramides was performed by adding 250 nmol of Cer 35:1;2 and GlcCer 30:1;2 (Avanti Polar Lipids. Alabaster, AL, USA) as standards to the lipid extracts. Quantification of sphingolipids relied on extracted intensities of specific structural fragments of the long chain base (LCB): m/z 264.2 (for LCB 18), 236.2 (for LCB 16), 234.2 (for LCB 16:1), 208.2 (for LCB 14) and 206.2 (for LCB 14:1).
| + | see http://dev.biologists.org/content/137/21/3675.long |
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| − | ====MFQL queries====
| + | ===MFQL queries=== |
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| | MFQL queries for ceramides, PE-ceramids and glucosylceramide (hexosyl ceramides) in positive mode: | | MFQL queries for ceramides, PE-ceramids and glucosylceramide (hexosyl ceramides) in positive mode: |
Latest revision as of 16:33, 30 August 2012
Survival strategies of a sterol auxotroph
Materials and Methods
see http://dev.biologists.org/content/137/21/3675.long
MFQL queries
MFQL queries for ceramides, PE-ceramids and glucosylceramide (hexosyl ceramides) in positive mode:
