LX Reference Carvalho 2010
Queries, spectra and results
Materials and Methods
Top-down analysis of the fly total membrane lipidome was performed on an LTQ Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) equipped with a NanoMate robotic nanoflow ion source (Advion BioSciences, Ithaca, NJ, USA) as described previously (Schwudke et al., 2007a). MS/MS experiments on the LTQ Orbitrap and the QSTAR Pulsar i (MDS Sciex, Toronto, Canada) mass spectrometers were performed as described previously (Schwudke et al., 2007b). Absolute quantification of ceramides and hexosyl-ceramides was performed by adding 250 nmol of Cer 35:1;2 and GlcCer 30:1;2 (Avanti Polar Lipids. Alabaster, AL, USA) as standards to the lipid extracts. Quantification of sphingolipids relied on extracted intensities of specific structural fragments of the long chain base (LCB): m/z 264.2 (for LCB 18), 236.2 (for LCB 16), 234.2 (for LCB 16:1), 208.2 (for LCB 14) and 206.2 (for LCB 14:1).
MFQL queries
MFQL queries for ceramides, PE-ceramids and glucosylceramide (hexosyl ceramides) in positive mode:
