Difference between revisions of "LX Reference Ayciriex"

Revision as of 17:33, 24 February 2012

Queries, spectra and results

Materials and Methods

Yeast lipid extracts were prepared according to Ejsing et al. (2009) with slight modifications. Anionic glycerophospholipid species recovered in the 2:1 phase lipid extracts were detected by negative ion mode Fourier-transform mass spectrometry (FT MS) analysis by applying low m/z range scans (m/z 200–605) and high m/z range scans (m/z 505–1400) with target mass resolution of 100,000 (full-width at half-maximum [FWHM]) on a LTQ-Orbitrap mass spectrometer (Thermo Fisher Scientific, Lafayette, CO) equipped with the robotic nanoflow ion source TriVersa NanoMate (Advion Biosciences, Indianapolis, IN; Ejsing et al., 2006, 2009). The 2:1 phase lipid extracts were infused using methylamine (0.2 mM final in methanol). TAG, DAG, PC, and PE species recovered in the 15:1 phase lipid extracts were monitored by positive ion mode FT MS analysis (Ejsing et al., 2006; Schwudke et al., 2006, 2007) applying looped low m/z range scans (m/z 260–530) and high m/z range scans (m/z 500–1050) with target mass resolution of 100,000 (FWHM). The 15:1 phase lipid extracts were infused using ammonium acetate (7.5 mM final). Detected lipid species were identified and quantified using LipidXplorer (Herzog et al., 2011), as described in Graessler et al. (2009). The structures of detected lipid precursors were verified manually by MSn analysis. For NL experiments on TAG species, the experiments were performed on the AB SCIEX QTRAP 5500 system in direct infusion at 10 µl/min (AB Sciex, Foster City, CA). Please have a look into the article for a detailed description of the experiments: []

MFQL queries

• File:PC-positive-screen.mfql
• File:PE-positive-screen.mfql
• File:TAG-positive-screen.mfql
• File:DAG-positive-screen.mfql