FAQ
From LipidXplorer
Contents
- 1 Python is installed, LipidXplorer is installed, but clicking on lpdxGUI.pyw does nothing
- 2 When I click on a MFQL query in the LipidXplorer Wiki it opens a html page instead of the script
- 3 LipidXplorer does not find any lipid although there are for sure lipids in the sample
- 4 Why are +-0.5 in the double bond equivalents?
- 5 Is there a difference in quality between spectra recorded in 'profile' mode and 'centroid' mode?
Python is installed, LipidXplorer is installed, but clicking on lpdxGUI.pyw does nothing
Right click on LipidX
or lpdxGUI.pyw
. In the upcoming menu select Edit with IDLE
. This should open 2 windows. One is the Python Shell
and the other is the source code of lpdxGUI.pyw
. Click on the source code window and press F5
. If LipidX won't start then there will be an error message:
-
ImportError: DLL load failed: This application has failed to start because the application configuration is incorrect. Reinstalling the application may fix this problem.
:
- This error occurs because the C runtime library is not being installed correctly on your machine. (see [1]).
- Please install the following if you have a x32 processor or a x64 processor
When I click on a MFQL query in the LipidXplorer Wiki it opens a html page instead of the script
If you click on a *.mfql script the file menu opens first. Here you left-click again on the *.mfql script to finally open it. Or click with the right mouse button on it to "Save [it] As..." .
LipidXplorer does not find any lipid although there are for sure lipids in the sample
If your import file format is *.dta/*.csv check:
- if you acquired your sample in negative ion mode you have to add the preposition 'neg_' on your data folder. (see Reference)
Why are +-0.5 in the double bond equivalents?
LipidXplorer calculates the double bond equivalent with a mathematical formula. If it is an ion, it has one proton less or more. This gives a 0.5 in the calculation. In more detail:
- +0.5 if the ion is negative
- -0.5 if the ion is positive